CIRP

At lower temperatures, cell growth and gene expression are generally decreased. However, mammalian male germ cells proliferate and differentiate well at moderately low temperatures (32-35°C), and die at normal body temperature. These findings suggest that some genes may be expressed better at mild hypothermia. Interprotein is searching for partners that practically use it for manufacture of protein medicines or preparation of protein reagents (research use).

Genetic element that enhances transcription of CIRP gene at moderately low temperature (32 C) has been also identified. This element exists at upstream of CIRP gene and named mild cold response element (MCRE).

When human erythropoietin (EPO)-producing Chinese hamster ovary (CHO) cells were transfected with MCRE, the cells produced an increased amount of EPO in response to mild cold stimulation as compared with culture at an ordinary temperature (37°C).

We believe that this technology would be available for the production of protein medicines such as hematopoietic growth factors, enzymes, monoclonal antibodies and soluble receptors by mammalian cells including CHO cells. Extensive usage of this technology would realize a drastic cost down of the protein drugs, and result in those big price down and wider administration to patients.